Light Sheet Microscopy Vs Confocal - Web light sheet fluorescence microscopy (lsfm) illuminates a thin slice of a fluorescent sample and observes the emission from this plane perpendicularly 1. Both techniques involve laser scanning of a specimen to produce an image. Web light sheet fluorescence and laser scanning confocal microscopy are both used to acquire 3d image datasets with optical sectioning, however they differ significantly in imaging speed and resulting data quality. Web nevertheless, confocal microscopy remains superior for high resolution imaging of small features when compared to lightsheet microscopy. The main difference is that confocal microscopes use a series of pinholes to exclude light outside the focal plane. In consequence, a light sheet. As such, lightsheet microscopy is an excellent tool for measuring larger specimens where the drop in resolution compared to confocal imaging will have a negligible impact. This immediately raises the question of whether it would be possible to combine both strategies in one single system.
Web light sheet fluorescence and laser scanning confocal microscopy are both used to acquire 3d image datasets with optical sectioning, however they differ significantly in imaging speed and resulting data quality. This immediately raises the question of whether it would be possible to combine both strategies in one single system. Web light sheet fluorescence microscopy (lsfm) illuminates a thin slice of a fluorescent sample and observes the emission from this plane perpendicularly 1. Web nevertheless, confocal microscopy remains superior for high resolution imaging of small features when compared to lightsheet microscopy. Both techniques involve laser scanning of a specimen to produce an image. In consequence, a light sheet. As such, lightsheet microscopy is an excellent tool for measuring larger specimens where the drop in resolution compared to confocal imaging will have a negligible impact. The main difference is that confocal microscopes use a series of pinholes to exclude light outside the focal plane.